生物技术通报2015，Vol.31Issue(5)：73-77,5.DOI:10.13560/j.cnki.biotech.bull.1985.2015.05.012
适于双向电泳分析的酵母胞外蛋白提取方法
Procedure to Prepare Samples for Two-dimensional Electrophoresis of Secreted Proteins from Saccharomyces cerevisiae
杜维 1朴永哲 2黄玮 1谷月 1赵长新1
作者信息
- 1. 大连工业大学生物工程学院,大连 116034
- 2. 大连民族学院,大连 116600
- 折叠
摘要
Abstract
Saccraomyces cerevsiae FFC2144 was cultured in nitrogen base medium without protein. The secretory proteins of yeasts were extracted by ammonium sulfate precipitation, ultrafiltration and lyophilization-phenol extraction respectively. Extraction rates by 3 methods were calculated and the proteins were separated by two-dimensional electrophoresis. The proteins isolated were confirmed by MALDI-TOF-MS. The extraction rate by lyophilization-phenol method was 73.67% and 114 protein spots were obtained with the most protein spots and clearest electrophoretogram. Lyophilization-phenol method could be an ideal separation method for studying secretory proteomics.关键词
酿酒酵母/胞外蛋白/双向电泳/提取方法Key words
Saccharomyces cerevisiae/secretory protein/two-dimensional electrophoresis/extraction method引用本文复制引用
杜维,朴永哲,黄玮,谷月,赵长新..适于双向电泳分析的酵母胞外蛋白提取方法[J].生物技术通报,2015,31(5):73-77,5.
