生物技术通报2015，Vol.31Issue(5)：84-92,9.DOI:10.13560/j.cnki.biotech.bull.1985.2015.05.014
基于RNA-seq技术的楮头红转录组分析
Transcriptome Analysis of Sarcopyramis nepalensisvia RNA-seq Technology
摘要
Abstract
Transcriptome analysis of Sarcopyramis nepalensisleaves was performed via a newly developed high-throughput sequencing technology(Illumina RNA-seq). A total of 51 305 unigenes were generated with 921 nt of average length and 1 490 nt of unigene N50 after filtering and assembly of original reads. These unigenes from thede novo assembly were further annotated using BLAST and BLAST2GO softwares. A total of 40 532 unigenes annotated with databases of non-redundant protein sequence(Nr), non-redundant nucleotide(Nt), Swiss-Prot, Gene Ontology database(GO), Clusters of Orthologous Groups(COG)and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases available at NCBI as references. The proportion of unigenes annotated in Nr, Nt, Swiss-Prot, KEGG, COG and GO databases were 77.53%, 56.18%, 53.14%, 46.58%, 29.69% and 60.72%, respectively. Total 39 302 CDSs were obtained using blast in protein databases, and 2 065 CDSs were predicted using ESTscan software. KEGG pathway parsing revealed that 2 323(9.72%)unigenes were involved in biosynthesis of secondary metabolites(KO01110), and 78 unigenes encoding the cytochrome P450 family proteins were identified. These annotated information provided theoretical foundation for determining the vital genes involved in biosynthesis of secondary metabolites of medicinal plants.关键词
楮头红转录组/RNA-seqKey words
Sarcopyramis nepalensistranscriptome/RNA-seq引用本文复制引用
金红,焦根林,陈刚..基于RNA-seq技术的楮头红转录组分析[J].生物技术通报,2015,31(5):84-92,9.基金项目
深圳市城市管理局项目(201318) （201318）
