生物技术通报2015，Vol.31Issue(5)：167-172,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.05.026
新型脱氮菌Rhizobium radiobacter的分离鉴定及其硝化特征分析
Isolation and Identification of Novel Denitrifier StrainRhizobium radiobacterand Its Nitrifying Characterisation
摘要
Abstract
In order to screen and identify new strain with nitrification, a denitrifier was isolated from sewage treatment plant. The strain named N312 isolated by enrichment and plate culture was identified based on its morphology characteristics, 16S rDNA gene sequence analysis and Biolog. The conditions of medium composition were optimized by orthogonal experiment. The autotrophic denitrifier strain N312 was isolated and identified asRhizobium radiobacter, which was rod, Gram-negative, colony round and white. The results indicated that the nitrite removal efficiency by the strain could reach 100% after 6 days. The best medium was NaNO2 1.5 g, Na2CO3 1.5 g, Mg2SO4·7H2O 0.25 g, FeSO4·7H2O 0.2 g,KH2PO4 0.5 g. Strain N312 is a new autotrophic denitrifier with a highly research value.关键词
脱氮菌/16SrDNA/Biolog/正交试验/硝化速率Key words
denitrifier/16S rDNA/Biolog/orthogonal experiment/nitrification rate引用本文复制引用
罗小溪,高建忠,陈再忠,于永亮..新型脱氮菌Rhizobium radiobacter的分离鉴定及其硝化特征分析[J].生物技术通报,2015,31(5):167-172,6.基金项目
上海高校知识服务平台上海海洋大学水产动物遗传育种中心项目(ZF1206) （ZF1206）
