生物技术通报2015，Vol.31Issue(5)：179-185,7.DOI:10.13560/j.cnki.biotech.bull.1985.2015.05.028
红笛鲷(Lutjanus sanguineus)C型凝集素基因的克隆与组织表达分析
Cloning and Tissue Expression Analysis of C-type Lectin Gene from Humphead Snapper(Lutjanus sanguineus)
摘要
Abstract
According to conservative region of known C-type lectin(CTL)gene, a pair of degenerate primers were designed, and full length cDNA sequence of C-type lectin gene was amplified by RT-PCR and RACE PCR from spleen of humphead snapper,Lutjanus sanguineus (GenBank accession number: AGT37609)for the first time. The total cDNA sequence of the gene was 828 bp, consisting of a 663 bp open reading frame(ORF)and encoding 220 amino acids. The deduced amino acid sequence of humphead snapper CTL shared 30%-68% identities with other species CTLs. Phylogenetic analysis showed that humphead snapper CTL was clustered closely with mummichog, rock bream, and orange-spotted grouper CTL. Moreover, the mRNA expression levels in different tissues were analyzed by real time quantitative PCR. The result showed that humphead snapper CTL gene expressed in all tested tissues with highest level in liver, spleen and skin, moderate level in head kidney, kidney, stomach, intestine and muscle, and lowest level in heart and brain.关键词
红笛鲷/C型凝集素/基因克隆/组织表达分析Key words
Lutjanus sanguineus/C-type lectin/gene cloning/tissue expression analysis引用本文复制引用
蔡佳,张雪利,吴灶和,简纪常,鲁义善,冯东岳,焦茂兴..红笛鲷(Lutjanus sanguineus)C型凝集素基因的克隆与组织表达分析[J].生物技术通报,2015,31(5):179-185,7.基金项目
广东高校国际合作创新平台项目(2013gjhz0008),十二五国家科技支撑计划(2012BAD17B02),广东教育厅科技创新重点项目(2012CXZD0026) （2013gjhz0008）
