生物技术通报Issue(6)：93-99,7.DOI:10.13560/j.cnki.biotech.bull.1985.2015.06.034
河北区试小麦品种（系）DNA指纹图谱构建及遗传差异分析
Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Wheat Varieties(Lines)in Regional Test of Hebei
摘要
Abstract
In order to provide a reference for improving and innovating breeding materials, we used forty two pairs of SSR primers to construct DNA fingerprints and detect genetic diversity among 70 wheat varieties(lines), which participated in regional test of Hebei in 2009-2013. The results showed that 303 allelic were revealed on 42 SSR loci, 7.21 alleles were detected for each SSR locus, and the average PIC(polymorphism information content)value was 0.69. Moreover, the average gene diversity was 0.73, and the genetic similarity varied from 0.05 to 1.00 with the average of 0.28. These results indicated that there is a large genetic difference between the wheat varieties(lines)in regional test of Hebei. The cluster analysis divided the 70 wheat varieties(lines)into four groups and six subgroups by UPGMA method. These results show that the wheat varieties(lines)have similar genetic background in the same breeding institute or region. We should strengthen the introduction and utilization of foreign wheat germplasm resources.关键词
小麦/SSR标记/DNA指纹图谱/遗传差异Key words
wheat/SSR markers/DNA fingerprinting/genetic varieties引用本文复制引用
李宏博,庞斌双,刘丽华,刘阳娜,赵昌平,陈景堂..河北区试小麦品种（系）DNA指纹图谱构建及遗传差异分析[J].生物技术通报,2015,(6):93-99,7.基金项目
国家科技支撑计划（2014BAD01B09，2013BAD04B01），北京市农林科学院科技创新重大专项 （）
