生物技术通报Issue(6)：106-110,5.DOI:10.13560/j.cnki.biotech.bull.1985.2015.06.015
油菜素内酯基因BAS1根系表达载体的构建及烟草的遗传转化
Construction of Root Expression Vector of Brassinosteroid GeneBAS1 and Genetic Transformation into Tobacco
摘要
Abstract
In order to allow brassinosteroid geneBAS1overexpress in root, the root expression vector pCAMBIA2301-RB7-rbc with root promoter TobRB7 was constructed.Then inactivated gene BAS1 was integrated into the root overexpression vector pCAMBIA2301-RB7-BAS1-rbc, and they were transformed into tobacco byAgrobactrium tumefaciems infection. With the verification by PCR and RT-PCR, the target gene BAS1 was transformed into tobacco genome successfully, and expressed in root;the expression of brassinosteroid receptor kinase geneBRI1 in roots of transgenic plants was affected.关键词
BAS1基因/根系表达载体构建/转基因植株/表达Key words
BAS1 gene/construction of root expression vector/transgenic plants/expression引用本文复制引用
束红梅,郭书巧,巩元勇,倪万潮..油菜素内酯基因BAS1根系表达载体的构建及烟草的遗传转化[J].生物技术通报,2015,(6):106-110,5.基金项目
国家自然科学基金项目（31201139），国家转基因专项（2011ZX08005-001），江苏省自主创新基金项目［CX（14）2065］ （）
