生物技术通报Issue(6)：129-137,9.DOI:10.13560/j.cnki.biotech.bull.1985.2015.06.020
香蕉根际土壤解钾放线菌的筛选鉴定及解钾特性研究
Screening,Identification and Potassium-dissolving Characteristics of Potassium-dissolving Actinomycete in Banana Rhizosphere Soil
摘要
Abstract
According to the characteristics and mechanism of potassium-dissolving by potassium-dissolving bacteria, strains of the highly efficient and stable potassium-dissolving bacteria were isolated from the banana rhizosphere soil. Firstly by primary screening in potassium-dissolving separation culture medium and screening culture medium, a number of highly efficient potassium-dissolving bacteria were obtained. Then identifying the strain types and determining the potassium-dissolving ratio in laboratory, highly efficient strains of potassium-dissolving actinomycetes were gained. Finally, based on the morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis, the strains were identified, the potassium-dissolving ratios of them were measured under different conditions, and the potassium-dissolving characteristics were studied. After identifying the isolated 16 strains of efficient potassium-dissolving bacteria and measuring their potassium-dissolving ratios, finally a stable potassium-dissolvingStreptomycete M3-4 was obtained. The potassium-dissolving ratio reached the highest under the conditions: 120 h, 30℃, pH=6, the medium potassium mineral content was 5 g, shaking speed in 250 r/min, maltose as carbon source, and peptone as nitrogen sources. The strain isStreptomyces shaanxiensis and potassium-dissolving ratio is about 20%.关键词
香蕉根际土壤/放线菌/解钾率Key words
banana rhizosphere soil/actinomycete/potassium-dissolving ratio引用本文复制引用
陈宇丰,柯春亮,周登博,高祝芬,戚春林,张锡炎..香蕉根际土壤解钾放线菌的筛选鉴定及解钾特性研究[J].生物技术通报,2015,(6):129-137,9.基金项目
现代农业产业技术体系建设专项资金项目 （）
