生物技术通报Issue(6)：216-220,5.DOI:10.13560/j.cnki.biotech.bull.1985.2015.06.022
犬α干扰素在家蚕杆状病毒表达系统中的表达及其抗病毒活性的测定
Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay
摘要
Abstract
Canine interferon alpha(CaIFNα)was widely used in the prophylaxis and cure of canine diseases. The purpose of this study is to develop an efficient method to express CaIFNα. A canine interferon alpha gene in GenBank was optimized according to the codon bias of silkworm and synthesized, and then cloned into the baculovirus transfer vector pVL1393 to construct the recombinant plasmid pVL-CaIFNα. The pVL-CaIFNα was co-transfected with the BmBacmid DNA into silkworm cells and afterin vivo recombination. The recombinant virus was gathered and used to infect silkworm larvae. The hemolymph of infected larvae was collected for antiviral activity assay. The antiviral activity of expressed CaIFNα was examined on Madin-Darby canine kidney cells infected with the vesicular stomatitis virus expressing green fluorescent protein(VSV*GFP). The result showed that CaIFNα expressed in silkworm can inhibit the multiplication of VSV*GFP and the antiviral activity was about 1.78×106U/mL.关键词
犬α干扰素/家蚕杆状病毒表达系统/抗病毒活性检测Key words
canine interferon alpha/silkworm-baculovirus expression system/antiviral activity assay引用本文复制引用
李皓洋,胡小元,易咏竹,杨鑫,张志芳,李轶女..犬α干扰素在家蚕杆状病毒表达系统中的表达及其抗病毒活性的测定[J].生物技术通报,2015,(6):216-220,5.基金项目
国家高技术研究发展计划“863”计划（2011AA100603），国家重点基础研究发展计划“973”计划 （）
