山西农业大学学报（自然科学版）Issue(5)：449-453,5.
棉属三元杂种的合成及细胞遗传学鉴定
Synthesis and Cytogenetic Identification of Trispecific Hybrid in Cotton
摘要
Abstract
There are rich germplasm resources in Gossypium and wild cotton species possess many valuable agronomic traits which cultivated cotton always lacks .In order to introgress the useful traits from wild species G .australe and G . darwinii into cultivated species ,tetraploid G . hirsutum was crossed directly with C‐genome species G . australe as male parent ,creating a triploid hybrid .Chromosome doubling of this triploid hybrid leads to a allohexaploid which was then crossed with wild tetraploid species G . darwinii ,resulting in trispecific hybrid (G .hirsutum ,G . australe ,G . darwinii ) .The morphology characteristic and cytogenetic identification of trispecific hybrid was further investigated in this study .The hybrid morphology performed combining characteristics from three parents .Results indicated that the meiosis of trispecific hybrid was abnormal ,and 65% abnormal multispores appeared in the telophase II ,which fi‐nally developed into abortive pollen grains .The result also showed that there were only 18.3% normal pollen grains in this hybrid by statistic analysis .It leads to the sterility of trispecific hybrid F1 .This study not only creats new cotton germplasms from G .hirsutum ,G .australe ,G .darwinii with three parents characteristic .It also provides the main reason of sterility for this hybrid .关键词
陆地棉/澳洲棉/达尔文棉/三元杂种/形态学/减数分裂Key words
G .hirsutum/G .australe/G .darwinii Watt/Trispecific Hybrid/Morphology/Meiosis分类
农业科技引用本文复制引用
荣二花,李灵娇,杨娜,陈弟,吴玉香..棉属三元杂种的合成及细胞遗传学鉴定[J].山西农业大学学报（自然科学版）,2015,(5):449-453,5.基金项目
国家自然科学基金（No ．31171599，31301364）；山西农业大学科技创新基金（2014YZ04）；博士基金（2013YJ45）；博士后基金 （）
