摘要
Abstract
Objective To establish a method for simultaneously determining the plasma concentration in multiple antiepileptic drugs, such as carbamazepine( CBZ) ,phenobarbital( PB) ,nitrazepam( NTZ) and clonazepam( CNZ) using high performance liquid chromatog-raphy( HPLC) . Methods The serum samples were processed by a certain method,in which the chromatographic column was Nova-pak C18(150 mm ×3.9 mm, 4 μm),the mobile phase was composed of 0. 01 mol/L monopotassium phosphate buffer solution(pH 2. 15)and acetonitrile(29∶71),the monitoring wavelength was 223 nm at a flow rate of 1 ml/min, and the column temperature was set at 30 ℃. Results The residual impurities of the processed serum did not interfere with the tested drugs,which were well separated under the chromatographic conditions. The concentration had a stable linear relationship with the peak area in the range of 0. 01-10 mg/L(r>0. 999 0). Of all the drugs,recoveries of the method were all higher than 90% while the relative standard deviations(RSD)of within-day and between-day were both less than 10%. Conclusion This method was efficient,accurate,simple and practical,and could be applicable for therapeutic drug monitoring.关键词
高效液相色谱法/卡马西平/苯巴比妥/硝西泮/氯硝西泮/血药浓度Key words
high performance liquid chromatography/carbamazepine/phenobarbital/nitrazepam/clonazepam/plasma concentration分类
医药卫生
