山西医科大学学报Issue(3)：227-230,4.DOI:10.13753/j.issn.1007-6611.2015.03.009
Toll样受体2在口腔鳞癌中的表达及其作用机制
Expression of Toll-like receptor 2 in oral squamous cell carcinoma and its mechanism
摘要
Abstract
Objective To explore the expression of Toll-like receptor 2(TLR2)in oral squamous cell carcinoma(OSCC) and its role in the development of tumor. Methods The expression of TLR2 was detected in peripheral monocyte of 24 cases of OSCC, 24 cases of benign tumor and 24 normal controls by RT-PCR and Western blot. And the TLR2 expression in tumor tissues was detected by immuno-histochemical method. Freund’ s complete adjuvant( CFA) and incomplete adjuvant( IFA) were injected into mice to observe the sur-vival time of tumor-bearing mice and the tumor growth. Results The expression of TLR2 mRNA in peripheral monocyte was higher in OSCC than those in normal controls or in benign tumor(P<0. 05). And the TLR2 protein levels were also higher in tumor tissues of OSCC than in normal controls or in benign tumor(P<0. 05). The tumor weight was(1. 42 ± 0. 43)g in mice injected with CFA,(2. 94 ± 0. 87)g in mice injected with incomplete adjuvant(IFA) and (2. 47 ± 0. 69)g in blank control mice. The survival time of the tumor-bearing mice in CFA group was significantly prolonged compared with IFA group and blank group(P<0. 05). Conclusion TLR2 expression increases in peripheral monocyte and tumor tissues of OSCC patients,and TLR2 high expression significantly inhibit the pro-liferation of human tongue squamous cancer cells.关键词
口腔鳞癌/TLR2/免疫组化Key words
tongue squamous cell carcinoma/Toll-like receptor 2/immunohistochemistry分类
医药卫生引用本文复制引用
王海欣,吴景华,陈继科,郭谊,时建华..Toll样受体2在口腔鳞癌中的表达及其作用机制[J].山西医科大学学报,2015,(3):227-230,4.基金项目
唐山市科技局课题资助项目(14130262a) （14130262a）
