现代检验医学杂志Issue(5)：23-27,5.DOI:10.3969/j.issn.1671-7414.2014.05.002
鸭肝组织中DHBcAg表达水平定量免疫组织化学方法的建立
Establishment of the Quantitative Immunohistochemical Examination of DHBcAg in Duck Liver
摘要
Abstract
Objective To develop a quantitative immunohistochemistry assay for duck hepatitis B virus core antigen (DHB-cAg)in duck liver tissue.Methods By comparison with no repair antigen and repair antigen with high pressure,microwave and trypsin,the best solution of antigen retrieval was determined.By optimizing the parameter of image acquisition and de-ducting blank area,mean density of yellow areas was calculated using Image-Pro Plus 6.0 software.Using the assay devel-oped to determine the level of DHBcAg in liver tissue from duck infected by DHBV,anti-DHBV activity of DHBcMAb-TAT PTD conj ugate was examined.Results SABC method with no repair antigen was selected,which was better than other methods.DHBcAg expression in duck liver tissue could be objectively and accurately quantified by setting Image-Pro Plus 6.0 software parameters and calculating mean density of yellow areas.By comparison with the differences between mean densityat baseline of treatment and end of treatment,it was showed that DHBcMAb-TATPTD conjugate treatment dose-de-pendently reduced the levels of DHBcAg in liver tissue,which show that the assay developed could effectively evaluate the anti-DHBV activity of agent.Conclusion The immunohistochemistry assay developed in this study can objectively and accu-rately evaluate the level of DHBcAg in duck liver tissue.关键词
鸭乙肝病毒核心抗原/免疫组织化学/Image-Pro Plus 6.0软件/定量检测Key words
duck hepatitis B virus core antigen (DHBcAg)/immunohistochemistry/Image-Pro Plus 6.0 software/quantitative assay分类
医药卫生引用本文复制引用
王亚文,刘正稳,刘希,惠凌云,巩辉,张琳,马列婷,韩水平,王全颖,杨广笑..鸭肝组织中DHBcAg表达水平定量免疫组织化学方法的建立[J].现代检验医学杂志,2014,(5):23-27,5.基金项目
国家自然科学基金资助，项目批准号81101260。 （）
