现代检验医学杂志Issue(5)：40-41,45,3.DOI:10.3969/j.issn.1671-7414.2014.05.007
肺结核患者血清caspase-3活性研究
Serum Caspase-3 Activity in Tuberculosis Patients
摘要
Abstract
Objective To explore serum caspase-3 activity in pulmonary tuberculosis patients.Methods 38 tuberculosis pa-tients in Infectious Disease Department in the First Affiliated Hospital of Guangxi Medical University were recruited and the peripheral venous blood was collected in December,2010.Among them,sputum smear-positive patients which were first treated were 13,sputum smear-negative patients which were first treated were 15,10 cases were drug resistance.In addition, 22 unrelated healthy individuals from a routine health survey were enrolled as controls.Spectrophotometry was used to de-tect serum caspase-3 activity in tuberculosis patients,which was absorbancy-A of 4-nitroaniline generated by caspase-3.Re-sults The activity of caspase-3 in sputum smear-positive patients,sputum smear-negative patients,drug resistance patients, and healthy controls were 37.15±5.06,50.38±6.29,45.32±3.32 and 23.89±2.29,respectively.The caspase-3 activity was significantly different from each other (F=10.68,P<0.01).Compared with the healthy controls,the caspase-3 activity in sputum smear-positive patients (q=3.51,P=0.016),sputum smear-negative patients (q=7.5,P<0.001),drug resist-ance patients (q=5.34,P=0.001)was significantly different.There was no statistical difference among sputum smear-posi-tive patients,sputum smear-negative patients and drug resistance pulmonary tuberculosis patients (P>0.05).In the same way,there was no significant difference in male/female ratio[χ2=0.38 (DL=3),P=0.94]and mean age (F=0.82,P=0.49)among the four group.Conclusion Caspase-3 closely associated with the development of tuberculosis occurrence, which might promote apoptosis of cells through the activation of caspase-3 and produce the body immune process of tubercu-losis.关键词
肺结核/caspase-3/凋亡Key words
tuberculosis/caspase-3/apoptosis分类
医药卫生引用本文复制引用
赵英伦,胡溪源,李翠萍,周怡,李洪涛,何敏,罗蓉..肺结核患者血清caspase-3活性研究[J].现代检验医学杂志,2014,(5):40-41,45,3.基金项目
本研究受国家自然科学基金（No.81160141），广西自然科学基金（No.2012GXNSFBA053116）资助。 （）
