现代检验医学杂志Issue(5)：46-48,3.DOI:10.3969/j.issn.1671-7414.2014.05.009
TRAIL在原发免疫性血小板减少症患者外周血单个核细胞中的表达及临床意义
Increased TRAIL Expression in Peripheral Blood Mononuclear Cells from Patients with Primary Immune Thrombocytopenia and Its Clinical Significance
摘要
Abstract
Objective To explore TRAIL expression in patients with primary immune thrombocytopenia (ITP)and its role in the pathogenesis.Methods Peripheral Blood Mononuclear Cells (PBMCs)from twenty-eight patients with ITP and thirty healthy controls were obtained.TRAIL expression was determined using Real-time Quantitative Polymerase Chain Reaction (RT-PCR).Serum TNF-α,IFN-γ,IL-4 and IL-10 were detected using ELISA.Associations between TRAIL expression and clinical parameters were assessed.Results Compared to healthy controls,TRAIL expression was significantly increased in PBMC from patients with ITP (2.80±0.43 vs 1.00±0.24,t=19.72,P<0.001).Compared to the healthy controls,ex-pression of IFN-γand TNF-αfrom ITP patients were increased (52.43±11.04 pg/ml vs 23.26±8.15 pg/ml;69.14± 10.89 pg/ml vs 36.14±13.17 pg/ml,t=10.36~11.50,P<0.001)and expression of IL-10 were decreased (11.18±6.13 pg/ml vs 33.28±9.85 pg/ml,t=10.17,P<0.001)and there was no significant difference in IL-4 expression (35.75± 12.52 pg/ml vs 40.16±14.26 pg/ml,t=1.25,P>0.05).Furthermore,TRAIL expression was positively correlated with serum IFN-γ,TNF-αlevel (r=0.432,0.541,P<0.05),and negatively correlated with IL-10 and PLT (r=-0.424,-0.553,P<0.05).Conclusion Rur results suggest that TRAIL may be involved in the pathogenesis of ITP.关键词
原发免疫性血小板减少症/外周血单个核细胞/TRAILKey words
primary immune thrombocytopenia/peripheral blood mononuclear cells/TRAIL分类
医药卫生引用本文复制引用
叶辛,张蕾,谷明莉,张薇薇,邓安梅,钱宝华..TRAIL在原发免疫性血小板减少症患者外周血单个核细胞中的表达及临床意义[J].现代检验医学杂志,2014,(5):46-48,3.基金项目
973课题（2013CB531606），国家自然科学基金（81273282，81202353，30972730），上海科委基金（11JC1410902），杨浦区人才发展资金（鼎元资金），吴阶平基金（320.6750.13147），长海医院基金（CH125530300）。 （）
