现代检验医学杂志Issue(5)：64-66,3.DOI:10.3969/j.issn.1671-7414.2014.05.014
类风湿关节炎患者外周血单个核细胞microRNA-206表达水平的变化及意义
Level of MicroRNA-2 0 6 in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis and Their Significance
摘要
Abstract
Objective To explore the role of microRNA-206 (miR-206)in peripheral blood mononuclear cells (PBMC)from rheumatoid arthritis (RA)patients.Methods 27 patients with RA and 25 healthy controls were enrolled into the current study.Human peripheral blood mononuclear cells (PBMCs)were isolated by standard density-gradient centrifugation over Ficoll-Hypaque solution in rheumatoid arthritis and healthy control volunteers.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,then the RNA was transcribed reversely into cDNA.The expression of mi-croRNA-206 and Kruppel-like factor 4 (KLF4)and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative PCR (qRT-PCR)method.Student’s unpaired t-test and spearman correlation were used for statistica1 analysis. Results The expression of miR-206 in the PBMCs of RA patients was significantly decreased compared with that of the healthy controls (0.056±0.019 vs 0.138±0.057,t=3.103,P<0.01),The levels of KLF4 and RORγt mRNAin the PB-MCs of RA patients were increased significantly verves those of the healthy controls(0.604±0.183 vs 0.098±0.027,t=6.651,P<0.01;0.583±0.271 vs 0.069±0.018,t=7.438,P<0.01),Furthermore,a negative correlated between the ex-pression of miR-206 and KLF4 or RORγt mRNA in RA patients (r=-0.639,P<0.01;r=-0.842,P<0.01).Conclusion These results indicated that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Th17 cells in RA patients.关键词
类风湿关节炎/microRNA-206/锌指样转录因子4/孤儿核受体γtKey words
rheumatoid arthritis/micro RNA-206/kruppel-like factor 4/RORγt分类
医药卫生引用本文复制引用
徐庆雷,朱宝林,马小波,张巍,刘玲玲,王胜军,陈建国..类风湿关节炎患者外周血单个核细胞microRNA-206表达水平的变化及意义[J].现代检验医学杂志,2014,(5):64-66,3.基金项目
江苏省卫生厅科研资助项目（Z201313）。 （）
