现代检验医学杂志Issue(6)：78-80,3.DOI:10.3969/j.issn.1671-7414.2014.06.025
脑脊液流式细胞术在检测中枢神经系统白血病中的应用
Value of Cerebrospinal Fluid Detection with Flow Cytometry in the Diagnosis of Central Nervous System Leukemia
孙雪静 1万岁桂 1刘聪燕 1贺景娟 1刘艳 1张维 1赵弘 1徐娟1
作者信息
- 1. 首都医科大学宣武医院血液科，北京 100053
- 折叠
摘要
Abstract
Objective To explore the clinical diagnostic significance of cerebrospinal fluid (CSF)detection with flow cytome-try (FCM)in central nervous system leukemia (CNS-L).Methods The 1 9 5 cerebrospinal fluid (CSF)samples were ana-lyzed using four-color flow cytometry by CD45/SSC secondary gating strategy and detected by conventional cell morpholo-gy.Results The results showed that 24 (33.80%)patients had abnormal cells by flow cytometry and 15 (19.72%)pa-tients had abnormal cells by conventional cell morphology in 71 Non-Hodgkin’s lymphoma (NHL)patients,respectively. The difference was statistically significant (P=0.022).The results showed that 13 (22.41%)patients had abnormal cells by flow cytometry and 1 2 (1 8.9 7%)patients had abnormal cells by conventional cell morphology in acute lymphoma leuke-mia (ALL)patients,respectively.The difference was no statistically significant (P=1.000).The results showed that 4 (5.9 7%)patients had abnormal cells by flow cytometry and 4 (5.9 7%)patients had abnormal cells by conventional cell morphology in acute nonlymphocytic leukemia (ANLL)patients,respectively.The difference was no statistically significant (P=1.000).Conclusion Cerebrospinal fluid (CSF)detection with flow cytometry (FCM)may be helpful in the diagnosis of central nervous system leukemia in NHL.It has higher sensitivity and is an important supplement to CSF conventional cell morphology.关键词
流式细胞术/脑脊液/免疫分型/中枢神经系统白血病Key words
flow cytometry/cerebrospinal fluid/immunophenotyping/central nervous system leukemia分类
医药卫生引用本文复制引用
孙雪静,万岁桂,刘聪燕,贺景娟,刘艳,张维,赵弘,徐娟..脑脊液流式细胞术在检测中枢神经系统白血病中的应用[J].现代检验医学杂志,2014,(6):78-80,3.
