现代检验医学杂志Issue(6)：84-86,90,4.DOI:10.3969/j.issn.1671-7414.2014.06.027
深圳地区儿童腹泻A组轮状病毒检测和基因型分析
Detection and Genotyping of Group A Rotavirus from Children with Diarrhea in Shenzhen
王琼 1张银辉 1何涛君 1黄烈 1杨来智 1吴润香 1陆学东1
作者信息
- 1. 广东医学院附属福田医院检验医学部，广东深圳 518033
- 折叠
摘要
Abstract
Objective To investigate the prevalence and genotype characteristics of group Arotavirus among children under 5 years of age with diarrhea in Shenzhen.Methods 413 fecal specimens were collected from children with acute diarrhea in Futian hospital from August 2007 to February 2009 in Shenzhen.Reverse transcription PCR(RT-PCR)were used to detect group A rotavirus.G and P genotyping were conducted to the positive specimens by using nested RT-PCR to amplify VP7 and VP4 gene.Results Group A rotavirus was detected in 217 of 413 specimens with the positive rate of 52.5%.The main epidemic season for rotavirus was from October to January in Shenzhen.The majority of children suffered rotavirus were un-der 2 years of age which account for 91.2% (198/217)of all rotavirus infections.The prevalence of G genotype was G3 (52.1%),G1 (31.3%),Gmix (6.0%),G2 (1.8%)and G9(1.4%)respectively.The prevalence of P genotype was P[8] (85.7%),P[4](6.0%)and Pmix (1.4%)respectively.G3P[8]and G1P[8]were the most common G and P genotype combinations accounting for 47.5% and 30.4% of rotavirus infections,respectively.Conclusion Group A rotavirus was the major pathogen of diarrhea among children in Shenzhen.G3P[8]and G1P[8]were the predominant genotypes from 2007 to 2009.G mix and P mix genotype were not uncommon in this region.关键词
轮状病毒/腹泻/儿童/基因分型Key words
rotavirus/diarrhea/children/genotyping分类
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王琼,张银辉,何涛君,黄烈,杨来智,吴润香,陆学东..深圳地区儿童腹泻A组轮状病毒检测和基因型分析[J].现代检验医学杂志,2014,(6):84-86,90,4.
