摘要
Abstract
Objective To make a preliminary study on the results from qualitative and quantitative detection of low concentra-tion hepatitis B surface antigen(HBsAg).Methods 85 HBsAg low concentration serum samples (ELISA method test re-sults were 0.6<S/CO≤3.0),which collected in Hunan Wantwant Hospital from 2013 October to 2014 April,were retested by chemilumiescence microparticle immunoassay (CMIA)and time-resovled fluorescence immunoassay (TRFIA)methods at the same time respectively.The derermination of low concentration HBsAg positive rates of the three methods were divided into 0.6<S/CO≤1.0,0.6<S/CO≤3.0 two groups using chi-square test.The pairwise comparison of HBsAg contens of the three methods were divided into 0.6<S/CO≤1.0,1.0<S/CO≤2.0,2.0<S/CO≤3.0,0.6<S/CO≤3.0 four groups using paired-samples t test.Adopted correlation coefficient t test in the analysis of the correlation of three different methods test results.Results ①The positive detection rate showed no statistically difference between CMIA (64.0%,89.4%)and TRFIA (54.0%,87.1%)in 0.6<S/CO≤1.0 group (χ2=0.333,P>0.05)and 0.6<S/CO≤3.0 group (χ2=0.227,P>0.05).The positive detection rate showed statistically difference between ELISA (70.6%)and CMIA,TRFIA in 0.6<S/CO≤3.0 group (χ2=9.412,6.907,P<0.05).The positive detection rate was CMIA>TRFIA>ELISA.②The contents of HBsAg showed statistically difference among 0.6<S/CO≤1.0,1.0<S/CO≤2.0,2.0<S/CO≤3.0 three groups in addi-tion to between 2.0<S/CO≤3.0 group of ELISA and CMIA,ELISA and TRFIA (t=1.743~3.671,P<0.05).The con-tents of HBsAg showed statistically difference among 0.6<S/CO≤3.0 group (t=2.053,2.766,4.459,P<0.05).The con-tent was CMIA>TRFIA>ELISA.③There was a positive correlation between the three methods of HBsAg content (t=2.939,2.928,60.915,P<0.05).The correlation between CMIA method and TRFIA method was the best (r=0.989).Con-clusion CMIA was the first choice for testing low concentration HBsAg,TRFIA was the second.For the specimens of the low concentration HBsAg detected by ELISA should be suggested to clinical and retested by CMIA or TRFIA in order to a-void missing detection.And it was not recommended to clinical that different methods of quantitative or half-quantitative re-sults were transverse compared in order to avoid misdiagnosis.关键词
低浓度乙肝表面抗原/酶联免疫吸附试验/化学发光微粒子免疫分析法/时间分辨荧光免疫法Key words
low concentration hepatitis B surface antigen/enzyme-linked immunosorbent assay/chemiluminescence microparti-cle immunoassay/time-resolved fluorescence immunoassay分类
医药卫生
