现代检验医学杂志Issue(1)：4-7,4.DOI:10.3969/j.issn.1671-7414.2015.01.002
分子生物学方法在肠球菌耐药性研究中的应用
Application of Molecular Biology in Investigation of Resistance in Enterococci
王珊 1吕媛1
作者信息
- 1. 北京大学第一医院临床药理研究所，北京 100191
- 折叠
摘要
Abstract
Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to re-sistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent lit-eratures on resistance of Enterococci and a systemic analysis was conducted.Results Common detecting methods include polymerase chain reaction (PCR),pulsed field gel electrophoresis (PFGE),multilocus sequence typing (MLST)and South-ern blot.There also exist less widely-used methods such as pyrosequencing and genechip technique,which may prove effi-cient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.关键词
肠球菌/耐药性/分子生物学/万古霉素耐药肠球菌Key words
enterococci/resistance/molecular biology/vancomycin-resistant enterococci分类
医药卫生引用本文复制引用
王珊,吕媛..分子生物学方法在肠球菌耐药性研究中的应用[J].现代检验医学杂志,2015,(1):4-7,4.
