现代检验医学杂志Issue(2)：11-15,5.DOI:10.3969/j.issn.1671-7414.2015.02.004
病毒性脑炎患儿脑脊液人类鼻病毒检测的价值
Value of Human Rhinovirus Detected in Cerebrospinal Fluid of Children with Viral Encephalitis
高珺 1王琼 2林广裕 3陈洁玲 3陆学东2
作者信息
- 1. 广东医学院，广东湛江 524023
- 2. 广东医学院附属福田医院医学检验部，广东深圳 518033
- 3. 汕头大学医学院第二附属医院儿科，广东汕头 515041
- 折叠
摘要
Abstract
Objective To understand the pathogen of viral encephalitis(VE)in children,and establish a rapid and specific method for detecting human rhinovirus(HRV),and investigate the correlation between HRV and viral encephalitis(VE). Methods 169 CSF specimens were collected from children with convulsions and fever,who were admitted to the pediatric intensive care unit (PICU)of Second Affiliated Hospital of Shantou University Medical College between January 2012 and December 2012.Nested RT-PCR was used to detected HRV in CSF specimens,and the positive PCR products were se-quenced,then analyzed and constructed the phylogenetic tree by software.Results 39 (23.1%)out of 169 samples were HRV positive.Among them,148(87.6%)children were below 5 years old.The detection rate of HRV increases from July to September,and reached its highest point in September.Sequence analyzed showed that the 39 HRV positive specimens inclu-ding 18(46.1%,18/39)positive for HRV-A,7(17.9%,7/39)positive for HRV-B,14(35.9%,14/39)positive for HRV-C. There were 8 out of 28 VE cases were detected in HRV,including 3(50%,3/6)positive for HRV-C.Conclusion HRV could be detected in CSF specimens by nested RT-PCR,including three types of HRV,combined with clinical symptoms con-sidered that HRV may be one of the VE pathogen.关键词
鼻病毒/病毒性脑炎/儿童/巢式逆转录聚合酶链反应Key words
human rhinovirus/viral encephalitis/children/nested reverse transcription-polymerase chain reaction分类
医药卫生引用本文复制引用
高珺,王琼,林广裕,陈洁玲,陆学东..病毒性脑炎患儿脑脊液人类鼻病毒检测的价值[J].现代检验医学杂志,2015,(2):11-15,5.
