现代检验医学杂志Issue(3)：28-31,4.DOI:10.3969/j.issn.1671-7414.2015.03.008
利用实时细胞分析系统检测人和大鼠气道平滑肌细胞倍增时间的研究
Research on Detecting Doubling Time of Human and Rat Airway Smooth Muscle Cells with A Real Time Cellular Analyzer
摘要
Abstract
Objective To detect the doubling time of airway smooth muscle cells of human and rat by the xCELLigence in-strument,a real time cellular analyzer.Methods The airway smooth muscle cells were separated by collagenase-pancreatin digestion from the rat airway.Then,added to the different holes of E-plate of xCELLigence instrument with the planting destiny of 3 000 cells/well.and so were the human airway smooth muscle cells.The E-plate was then placed on the xCELLi-gence instrument to monitor cell proliferation for 100 hours to calculate the doubling time by using the RTCA Software Package 2.0 software.Results The doubling time of human airway smooth muscle cell calculated by the real time cellular analyzer was 23.96±0.47 h,which was consistent with the data provided by the reference.The doubling time of rat airway smooth muscle cell was 18.62±0.15 h,and the computational process was simple,time-saving and also effective.Conclusion The xCELLigence instrument can be used to calculate doubling time of airway smooth muscle cells of human and rat, which provides experimental methods and reference data for the basic respiratory disease research.关键词
倍增时间/气道平滑肌细胞/细胞实时分析Key words
doubling time/airway smooth muscle cells/real time cellular analysis分类
医药卫生引用本文复制引用
王文倩,王宇,徐玉东,杨永清,尹磊淼..利用实时细胞分析系统检测人和大鼠气道平滑肌细胞倍增时间的研究[J].现代检验医学杂志,2015,(3):28-31,4.基金项目
国家自然科学基金项目（8117334；81173332；81473760）；上海市卫生系统优秀青年人才培养计划（XYQ2013081）；上海市中医药事业发展三年行动计划重大研究项目（ZYSNXD-CC-ZDYJ039）。 （）
