现代检验医学杂志Issue(3)：71-74,4.DOI:10.3969/j.issn.1671-7414.2015.03.020
幽门螺杆菌重组蛋白 OipA6间接 ELISA 法的建立
Establishment of An Indirect ELISA Method with Helicobacter Pylori Recombinated Antigene OipA6 as Coating Antigen
摘要
Abstract
Objective To establish an indirect ELISA method with Helicobacter pylori (HP)recombinated antigene OipA6 as coating antigen after prokaryotic expression and purification.Methods The ELISA plate was coated with Hp OipA6 recom-binant protein as antigen.Serum specimens and enzyme labeled anti human IgG were added.The reaction conditions was op-timized.The results of indirect ELISA were compared with the detection of oipA gene.Results When Hp OipA6 recombi-nant protein was 1.0 mg/ml,the condition of coating was 2~8℃ one night,the dilution of serum specimens was 1∶100 and enzyme labeled anti human IgG was 1∶4 000,the incubation time of enzyme labeled anti human IgG was 40 min,the chro-mogenic time was 15 min,the result of ELISA was the best.The sensitivity and specificity of the indirect ELISA were both higher than the detection of oipA gene.Conclusion It suggested that the indirect ELISA,in which Hp OipA6 recombinant protein were coated,may be an appropriate assisted method in detection of high virulent Hp infection.关键词
幽门螺杆菌高毒株/OipA6 重组抗原/间接 ELISA/辅助诊断Key words
high virulent Hp/oipA6 recombinant protein/indirect ELISA/diagnostics分类
医药卫生引用本文复制引用
李妮,佘菲菲..幽门螺杆菌重组蛋白 OipA6间接 ELISA 法的建立[J].现代检验医学杂志,2015,(3):71-74,4.基金项目
陕西省教育厅科研计划项目（项目编号14JK1626）；2014大学生创新创业计划项目（项目编号1635）。 （）
