陕西医学杂志Issue(5)：522-525,4.DOI:10.3969/j.issn.1000-7377.2014.05.003
采用IV型胶原酶建立小鼠脑出血模型
An animal model of collagenase type IV-induced intracerebral hemorrhage in mice
摘要
Abstract
Objective:To establish a typical mouse model of intracerebral hemorrhage (ICH) .Methods :Intracerebral hemorrhage in mice were generated by stereotaxically injecting different dose of collagenase type Ⅳ into the left corpus striatum .Every mouse received a volume of 0 .05μl collagenase type Ⅳ .Neurological scores were as-sessed and recorded 24 hours after injection .After behavioral testing the brains were processed for routine histologi-cal examination .Light microscope observation to determine if the animal model was successful .Results:Mice showed the motor dysfunction after operation .Morphology confirmed that the hematoma formed at the left corpus striatum ,and within a certain range ,the size of hemorrhage and the development of symptoms of hemiplegia was re-lated .Different individuals of the same unit of collagenase type Ⅳmaking the volume and location of hematoma was same .Conclusion :Intracerebral hemorrhage can be induced in mouse by injecting collagenase type Ⅳ into the corpus striatum of the brain .This is a useful economical and reproducible method to make intracerebral hemorrhage .ICH model was made by intracerebral injection of 0 .02U type IV collagenase can meet our experimental requirements .关键词
脑出血/化学诱导/脑出血/病理学/胶原酶/模型 ,动物/小鼠Key words
Intracerebral hemorrhage/chemically induced/Intracercbral hemorrhage/pathology/Colla-genase Models/Animal Mice分类
医药卫生引用本文复制引用
谭敬,肖新莉,陈新林,徐曦,高燕凤,刘勇..采用IV型胶原酶建立小鼠脑出血模型[J].陕西医学杂志,2014,(5):522-525,4.基金项目
国家自然科学基金（No30500575）；西安交通大学医学院第一附属医院科研基金 （）
