陕西医学杂志Issue(11)：1453-1455,1459,4.DOI:10.3969/j.issn.1000-7377.2014.11.004
顺铂作用下卵巢癌细胞株CP70中Eps8表达变化研究
Changes of Eps8’s expression in ovarian cancer cell strain CP70 under the action of cisplatin
摘要
Abstract
Objective:To observe the expression of tumor associated antigen Eps8(epidermal growth fac‐tor receptor pathway substrate 8 ,Eps8)in ovarian cancer cell strain A2780 ,CP70 ,SKOV3 and SKOV3/DDP ,screen out the cell strain CP70 which is at a high level in Eps8’s expression ,observe the changes of Eps8 protein in Cell CP70 under the action of Cisplatin(DDP) ,and discuss the role of Eps8 in chemotherapy resistance in ovarian cancer . Methods :Detected the Eps8’s expression level of mRNA in ovarian cancer cell strain by real‐time Fluorescence Quantitative PCR ,and using Western blot method to detect Eps8 protein expression level in order to screen out the high‐expression Cell CP70 .And then the Western Blot was used to detect the changes in Eps8’s expression in Cell CP70 after treatment of 24 and 48 hours with different concentration of DDP .Result :Eps8 protein’s expression was significantly elevated with the increase of drug concentration and effect time .Conclusions :Eps8 protein’s expression will increase after the cisplatin treatment on Cell CP70 ,which is also dependent on dose and time of DDP treatment . Besides ,the DDP could induce the increase of Eps8’s expression in Cell CP70 .So we can conclude that Eps8 can play an important role in the formation mechanism of cisplatin resistance in ovarian cancer .关键词
表皮生长因子受体通路底物8/CP70细胞/顺铂/药理学/卵巢肿瘤/免疫学Key words
Epidermal growth factor receptor pathway substrate 8/CP70 cells/Cisplatin/pharma-cology/Ovarian neoplasms/immunology分类
医药卫生引用本文复制引用
王冰,杨勇,辛晓燕..顺铂作用下卵巢癌细胞株CP70中Eps8表达变化研究[J].陕西医学杂志,2014,(11):1453-1455,1459,4.基金项目
国家自然科学基金项目 （）
