陕西医学杂志Issue(2)：134-137,4.DOI:10.3969/j.issn.1000-7377.2015.02.002
PIK3R1对多发性骨髓瘤细胞侵袭转移的影响及其机制初探
Experimental study on targeting PIK3R1 by RNAi suppressing Multiple Myeloma invasion and metastasis in vitro
摘要
Abstract
Objective:To observe the inhibitory effects on the invasion and metastasis of Multiple Myelo‐ma RPMI8226 cells line by small hairpin RNA targeting PIK3R1 in vitro .Methods :The recombinant plasmid vector expression vector which contained PIK3Rl shRNA was transfected into RPMI8226 cells .Real‐time PCR and Western blot were used to detect the expression of P1K3Rl .ELESA was used to measure the change in the MMP‐2 /MM P‐9 expression .The invasion ability of the tumor cells was examined by Transwell tests .Resuits :pGenesil‐1 .1‐PIK3R1 mediated shRNA targeting PIK3R1 dramatically down‐regulated their expression in RPMI8226 cells .MMP‐2 and MM P‐9 were downregulated ,and TIMP‐2 was upregulated .The extracellular levels of MMP‐2 and MMP‐9 were de‐creased .Transwell showed that the number of cells invading through the matrigel in control ,nonsense sequence . And pGenesil‐1 ,1‐PIK3R1 transfection groups were 115 .5 ± 3 .9 ,112 .8 ± 6 .0 ,and 73 .7 ± 4 .0 respectively .Conclu‐sion :ShRNA targeting PIK3R1 down‐regulated significantly their expression in a sequence‐specific manner ,and in‐hibited the invasion of Multiple Myeloma RPMI8226l cells in vitro .关键词
多发性骨髓瘤/肿瘤转移/基因治疗/PIK3R1Key words
Multiple myelom/Neoplasm metastasis/Gene therapy/PIK3R1分类
医药卫生引用本文复制引用
吴迪,刘亚林,王梦昌..PIK3R1对多发性骨髓瘤细胞侵袭转移的影响及其机制初探[J].陕西医学杂志,2015,(2):134-137,4.基金项目
陕西省科技攻关项目 （）
