陕西医学杂志Issue(7)：774-778,5.DOI:10.3969/j.issn.1000-7377.2015.07.002
大骨节病、骨关节炎软骨细胞分泌IL-1β、TNF-α及透明质酸对其影响实验研究
The effect of hyluronic acid on the expression of IL-1β,TNF-α in chondrocytes of Kashin-beck disease and osteoarthritis cultured in vitro
摘要
Abstract
Objective:To investigate the effect of HA on the expression of IL‐1β,TNF‐αin KBD and OA chondrocytes cultured in vitro to understand the effect and mechanism of HA on the treatment for KBD and OA . Method:We selected the articule cartilage sample of KBD and OA patients according to clinical diagnosis for KBD and OA .Chondrocytes of KBD ,OA and normal control were separated and cultured in vitro .Then varying dosages of HA were administered to chondrocytes ,100 μg/ml(H100 group)、500μg /ml(H500 group)and negative control (0 .00 μg /ml ,H0 group) ,and the expression of IL‐1β,TNF‐αwere detected by means of radio‐immunologic analy‐sis .Result :Compared with normal control group ,the IL‐1β,TNF‐α were higher in KBD and OA .after administed with HA ,the expression of IL‐1β,TNF‐αwere decreasing accordingly .there is no stastic signifcance in TNF‐αex‐pression .Conclusion :IL‐1β,TNF‐αwere higher in KBD and OA ,and the HA have the tendcency to decrease the ex‐pression of IL‐1β,TNF‐α.关键词
透明质酸/大骨节病/骨关节炎/软骨细胞/白细胞介素-1β 肿瘤坏死因子-αKey words
Hyaluronic acid/Kashin-beck disease/Osteoarthritis/Chondrocytes/Interleukin-lbeta/Tumor necrosis/factor-alpha分类
医药卫生引用本文复制引用
高宗强,郭雄,陈君长,段琛,马玮娟,顾其胜..大骨节病、骨关节炎软骨细胞分泌IL-1β、TNF-α及透明质酸对其影响实验研究[J].陕西医学杂志,2015,(7):774-778,5.基金项目
科技部国际合作重点专项（2006DFA33610）国家自然科学基金重点资助项目（30630058）陕西省社会发展公关资助项目 （）
