实用肝脏病杂志Issue(5)：519-522,4.DOI:10.3969/j.issn.1672-5069.2014.05.018
草苁蓉乙醇提取物对 NAFLD 大鼠肝脏炎性因子表达的影响
Impact of boschniakia rossica ethanol extract on hepatic expression of inflammatory cytokines in rats with NAFLD
何松美 1刘霞 2吴煜良 3刘旭明 1殷思纯 1龙尧4
作者信息
- 1. 523008 广东省东莞市人民医院感染病科
- 2. 广东医学院基础学院生理科学实验室
- 3. 523008 广东省东莞市人民医院肿瘤科
- 4. 广东医学院附属医院感染病科
- 折叠
摘要
Abstract
Objective To investigate the effect of boschniakia rossica ethanol extract (BREE) on hydroxyproline (Hyp) and inflammatory cytokines in rats with non -alcoholic fatty liver diseases (NAFLD). Methods The model of NAFLD in rats was established by hight -fat diet administration. Rats were randomly divided into control,model and BREE treatment group(500 mg·kg-1·d-1). All rats were sacrificed to harvest serum and liver tissues at the end of eighth week. Serum levels of AST,ALT and reduced glutathione (GSH) were measured,and the levels of interleukin (IL)-1β,tumor necrosis factor (TNF)-α and IL-6 in liver tissues were detected by ELSIA. HE staining and immunohistochemistry were performed for evaluation of liver pathological changes and the expression of NF-kB/p65 in liver tissues,respectively. Results Serum levels of ALT [(39.47± 20.26) U/L] and AST [(46.48 ± 18.52) U/L] in rats subjected to BREE treatment significantly decreased as compared to in the model group [(79.32±19.05)U/L and (88.35±17.46) U/L,respectively,P﹤0.01];Serum GSH levels in BREE-treated rats [(349.43±45.52) mg/L] significantly increased than in the model [(265.38±28.57) mg/L,P﹤0.01];The positive rates of NF-kB/p65 [(15.49±4.78)%] and Hyp contents [(14.28±3.08) mg/g] in liver tissues in BREE-treated rats significantly decreased than in the model [(87.54±6.59)% and (35.47±4.53)mg/g, respectively,P﹤0.01];the IL-1β,TNF-α and IL-6 levels [(34.51±5.61) pg/mL,(45.37±7.03) pg/mL,(18.98±5.04) pg/mL,respectively] in liver tissues in BREE-treated rats were significantly lower than in the model [(78.25±6.51) pg/mL,(85.64 ±6.25) pg/mL,(29.19 ±4.82) pg/mL,respectively,P ﹤0.01];BREE also significantly alleviated pathological changes in the liver. Conclusions BREE significantly improves liver function and collagen deposition in rats with NAFLD by down regulation of NF-κB/p65 and inflammatory cytokines in the liver.关键词
非酒精性脂肪性肝病/草苁蓉乙醇提取物/核转录因子κB/p65/炎性细胞因子Key words
Non-alcoholic fatty liver disease/Boschniakia rossica ethanol extract/Nuclear transcription factor κB/p65/Inflammatory cytokines引用本文复制引用
何松美,刘霞,吴煜良,刘旭明,殷思纯,龙尧..草苁蓉乙醇提取物对 NAFLD 大鼠肝脏炎性因子表达的影响[J].实用肝脏病杂志,2014,(5):519-522,4.
