石油实验地质Issue(5)：605-611,7.DOI:10.11781/sysydz201405605
典型油气藏上方甲烷氧化菌群的分子生物学解析
Molecular biological analysis of methanotrophic bacterial community above typical oil and gas reservoirs
摘要
Abstract
In this study, the community structure of aerobic methanotrophic communities were investigated at light hydrocarbon microseeps above a known oil band gas field in the Shengli Oilfield. Clone library analysis of the pmoA gene revealed major differences in the methanotrophic community composition between oil and gas fields and the surrounding soils. The relative abundance of methanotrophic Methylocystis and Methylosinus ( typeⅡ) in surrounding soils is obviously lower in oil and gas field soils than surrounding soils, ut the relative abundance of methanotrophic Methy-lococcus and Methylocaldum (type I) presents a reverse tread. Terminal restriction fragment length polymor-phism ( T-RFLP ) analysis confirmed that one distinct Methylococcus and Methylobacter-related group may dominate the community at hydrocarbon microseeps. The pmoA gene copy numbers determined by real time PCR also indicated that methane oxidizing microbial community anomalies above typical oil and gas reservoirs exist objectively.关键词
克隆文库/T-RFLP/pmoA基因/甲烷氧化菌/油气藏Key words
clone library/T-RFLP/pmoA gene/methanotrophic bacteria/oil and gas reservoirs分类
能源科技引用本文复制引用
汤玉平,高俊阳,赵克斌,任春,许科伟..典型油气藏上方甲烷氧化菌群的分子生物学解析[J].石油实验地质,2014,(5):605-611,7.基金项目
中国石化科技部项目（ P11058）资助。 （）
