实用医学杂志Issue(7)：1069-1072,4.DOI:10.3969/j.issn.1006-5725.2014.07.019
PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化
Overexpression of the tumor suppressor gene PTEN inhibits the phosphorylation of Akt in activated hepatic stellate cells in vitro
摘要
Abstract
Objective Using an adenoviral vector , the wild-type PTEN gene was transduced into activated hepatic stellate cell (HSC) in vitro and the phosphorylation status of Akt were investigated. Methods The wild type PTEN gene was transduced into activated HSC in vitro mediated by adenoviral vector. The expressions of PTEN and total Akt in HSC were measured by Western blot and Real-time fluorescent quantitation PCR. And the expressions of phosphorylated Akt (Thr308) in HSC was determined by Western blot. Results The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC in vitro. The over-expression of wild type PTEN resulted in the significant down-regulated expression of phosphorylated Akt (Thr308) in activated HSC (P < 0.01). But no significant defferences were found in the expression of total Akt in activated HSC at both transcriptional and translational levels(P>0.50). Conclusions The overexpression of wild-type PTEN can negatively regulate PI3K/Akt signaling transduction by inhibiting the phosphorylation of Akt in activated HSC in vitro.关键词
肝星状细胞/PTEN/Akt/信号转导Key words
Hepatic stellate cell/PTEN/Akt/Signaling transduction引用本文复制引用
郝礼森,刘玉龄,张晓岚,任昌镇,李立文,王静,莫艳波,边荣荣,魏月,张家琪..PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化[J].实用医学杂志,2014,(7):1069-1072,4.基金项目
河北省自然科学基金资助项目（编号H2013209327）；中国肝炎防治基金会天晴肝病研究基金资助项目 （）
