实用医学杂志Issue(9)：1363-1366,4.DOI:10.3969/j.issn.1006-5725.2014.09.007
阿托伐他汀通过PPARβ／δ途径下调老年大鼠心肌细胞IL-1β表达
Atorvastatin down-regulating IL-1β expression in myocyte of aging rat by PPARβ/δ signal channel
摘要
Abstract
Objective To investigate the correlation between atorvastatin inhibiting Interleukin-1β(IL-1β) expression and peroxisome proliferator activated receptor β/δ (PPARβ/δ) signal channel in myocyte of aging rat. Methods Primary culture of myocyte were got from aging rat. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GSK0660 group, whichwere treated respectively by Cell culture medium, dimethyl sulfoxide (DMSO), atorvastatin, atorvastin plus GSK0660. The expression level of IL-1βmRNA and protein was evaluated by RT-PCR and Western Blot respectively. Results (1)NO difference were found between control group and DMSO group in expression level of IL-1βmRNA and protein (P>0.05);(2)The expression level of IL-1βmRNA and protein in atorvastatin group were significantly lower than those of control group (P < 0.01); (3)Both mRNA and protein expression level of IL-1βin atorvastatin plus GSK0660 group were higher than those of atorvastatin group(P<0.05 or P<0.01), but still lower than those of control group(P<0.05). Conclusions Atorvastatin down-regulate the expression of IL-1βin aging myocytes by activating PPARβ/δsignal Channel.关键词
衰老/阿托伐他汀/过氧化物酶体增殖物激活型受体β/δ/白细胞介素-1β/大鼠/心肌细胞Key words
Aging/Atorvastatin/Peroxisome proliferator activated receptorβ/δ/Interleukin-1β/Rat/Myocyte引用本文复制引用
韩磊,叶平,李鸣皋..阿托伐他汀通过PPARβ／δ途径下调老年大鼠心肌细胞IL-1β表达[J].实用医学杂志,2014,(9):1363-1366,4.基金项目
国家自然科学基金资助项目 （）
