实用医学杂志Issue(11)：1760-1763,4.DOI:10.3969/j.issn.1006-5725.2014.11.024
白细胞介素24对急性白血病骨髓单个核细胞生长的影响
The effects of interleukin 24 on bone marrow mononuclear cells from adult with acute leukemias in vitro
摘要
Abstract
Objective The aim of this study was to observe the effects of interleukin-24 (IL-24) on the growth of acute leukemin bone marrow mononuclear cells (BMMNC) in vitro. And to investigate the mechanisms of IL-24 on acute leukemin BMMNC. Methods The group of acute lymphocytic leukemia (ALL) had 5 adults, the group of acute myeloid leukemia (ANLL) had 10 adults who did not receive any therapy. The acute leukemin BMMNC were separated from bone marrow. BMMNC were treated with IL-24 in vitro. The effects of IL-24 on acute leukemin BMMNC in vitro was detected by MTT assay. Apoptosis of acute leukemin BMMNC was measured by flow cytometry with AnnexinV-FITC staining. The expression of caspase-3 and bcl-2 in acute leukemin BMMNC was detected by RT-PCR. Results IL-24 can significantly inhibit the proliferation of acute leukemia BMMNC which was detected by MTT assay, and its effects were dose-dependent and time-dependent. After acute leukemia BMMNC were exposed to IL-24 for 48 h, the apoptotic rate of the group treated with IL-24 of 50 ng/mL was increased obviously. IL-24 increased the mRNA expression level of caspase-3, decreased the mRNA expression level of bcl-2 in acute leukemia BMMNC. Conclusion IL-24 can suppress the growth and induce apoptosis of acute leukemia BMMNC in vitro. IL-24 may inhibit the growth of acute leukemia BMMNC by up-regulating the expression of caspase-3 and down-regulating the expression of bcl-2.关键词
白细胞介素24/成人急性白血病/骨髓单个核细胞Key words
Interleukin-24/Adult acute Leukemin/Mononuclear引用本文复制引用
农卫霞,潘歆,魏娟,吴广胜..白细胞介素24对急性白血病骨髓单个核细胞生长的影响[J].实用医学杂志,2014,(11):1760-1763,4.基金项目
石河子大学自然科学一般项目 （）
