实用医学杂志Issue(12)：1875-1878,4.DOI:10.3969/j.issn.1006-5725.2014.12.007
apelin-APJ在慢性阻塞性肺疾病大鼠肺组织中的表达及其意义
Expression of apelin-APJ in chronic obstructive pulmonary disease and its significance
摘要
Abstract
Objective To detect the expression of apelin and angiotensin I receptor related protein (APJ) in rats with chronic obstructive pulmonary disease (COPD), and to explore the role of apelin-APJ system in the occurrence and development of COPD. Methods A rat model of COPD was established using the method of smoke exposure. SD rats were randomly divided into a control group and a COPD group. The rats in COPD group were exposed to secondhand cigarette smoke in a fume box twice a day for 4 months. Expressions of apelin mRNA and APJ mRNA in rat lung tissues were detected by real-time quantitative polymerase chain reaction (qRT-PCR). Expressions of apelin protein and APJ protein were determined by immunohistochemistry. Results The expressions of apelin mRNA and APJ mRNA in COPD group were decreased by 44% and 13% as compared with those in the control group. The mRNA expressions of apelin and APJ in rat lung tissues were negatively correlated with RV/(LV + S) (r = -0.454 and -0.448, P < 0.05), and positively related with FEV0.3/FVC (r = 0.529 and 0.475;P < 0.05). Apelin and APJ were mainly expressed in the epithelial cells of the bronch and lung, alveolar macrophages, vascular endothelial cells, and the membrane and cytoplasm of some alveolar wall cells. The expression of apelin and APJ protein was lower in COPD group than in the control group (P < 0.05). Conclusions The mRNA and protein expressions of apelin and APJ are decreased in the lung tissues in rats with COPD , which may be an important factor leading to the development of COPD. Apelin-APJ system might be a new target for prevention and treatment of pulmonary hypertension and right ventricular hypertrophy induced by COPD.关键词
肺疾病, 慢性阻塞性/Apelin/APJ/实时定量聚合酶链反应/免疫组织化学Key words
Pulmonary disease, chronic obstructive/Apelin/APJ/qRT-PCR/Immunohisto-chemistry引用本文复制引用
王嘉,张萍,李珠,孙佳玮,张彬,孙武装..apelin-APJ在慢性阻塞性肺疾病大鼠肺组织中的表达及其意义[J].实用医学杂志,2014,(12):1875-1878,4.基金项目
河北省卫生厅医学科学研究重点课题基金资助项目 （）
