实用医学杂志Issue(13)：2031-2034,4.DOI:10.3969/j.issn.1006-5725.2014.13.005
miR-19对肺腺癌A549细胞株迁移功能的影响
Effect of miR-19 on migration ability of lung adenocarcinoma cell line A549-Luc
摘要
Abstract
Objective To investigate the effects of miR-19 on the migration ability of lung adenocarcinoma cell line A549. Methods The expressions of miR-19 in lung epithelial cell line BEAS-2B and lung adenocarcinoma cell line A549-Luc were detected by qRT-PCR.The A549-Luc cell line which over-expressed miR-19 was established. The expression levels of miR-19 in A549/RFP+/H2B and A549/RFP+/m19 were identified by qRT-PCR. The morphology of A549/RFP+/m19 was observed,and the migration ability of A549/RFP+/m19 was detected by transwell migration assay. Results The expression levels of miR-19 differed significantly between BEAS-2B cells and A549-Luc cells (t = -20.954, P < 0.001). The lung adenocarcinoma cell line A549/RFP+/m19 which over-expressed miR-19 was successfully established. Changes in A549/RFP+/m19 cell morphology were found. As compared with A549/RFP+/H2B cells, A549/RFP+/m19 had an increased migration ability (P <0.01). Conclusions miR-19 enhances the migration ability of lung adenocarcinoma A549-Luc cells.关键词
肺腺癌/miR-19/迁移Key words
Lung adenocarcinoma/miR-19/Migration引用本文复制引用
鄢文,靳文,王昂,宋维舒..miR-19对肺腺癌A549细胞株迁移功能的影响[J].实用医学杂志,2014,(13):2031-2034,4.基金项目
国家自然科学基金青年科学基金项目（编号81302965）；广东省自然科学基金博士启动项目（编号S2013040014341）；广东省医学科研课题 （）
