实用医学杂志Issue(20)：3213-3215,3.DOI:10.3969/j.issn.1006-5725.2014.20.004
高迁移率族蛋白B1结合高级糖基化终产物受体可增加人支气管上皮细胞肿瘤坏死因子α的表达
High-mobility group box protein1 increases tumor necrosis factorαexpression in human bronchial epithelial cells
摘要
Abstract
Objective To investigate the effect of high-mobility group box protein1 (HMGB1) on the expression of TNF-αand its mechanism in 16HBE in vitro. Methods groups with different HMGB1 (0, 100, 500, 2 000 ng/mL) concentration was set; RAGE antagonizing groups were as control, HMGB1-2000ng, anti-RAGE and anti-RAGE+HMGB1. The changes of TNF-αmRNA and secretion were determined by quantitative PCR and ELISA. RAGE protein level was measured by western blotting. Results HMGB1 intervention and TNF-α expression of 16HBE presented a positive dose-dependent relationship. Thechanges of RAGE was HMGB1positively concentration dependent. In comparison with HMGB1 2 000 ng/mL group, anti-RAGE+HMGB1showed a remarkable reduction of TNF-α secretion. Conclusion In vitro, HMGB1 increases TNF-α expression in 16HBE with a dose-dependent manner through RAGE.关键词
高迁移率族蛋白B1 (HMGB1)/人支气管上皮细胞(16HBE)/肿瘤坏死因子-α(TNF-α)/高级糖基化终产物受体(RAGE)Key words
High mobility group box 1 (HMGB1)/human bronchial epithelial cells/tumor necrosis factorα(TNF-α)/receptor for advanced glycation end products(RAGE)引用本文复制引用
梁悦,侯长春,黄宏,邬丽红,陈一强..高迁移率族蛋白B1结合高级糖基化终产物受体可增加人支气管上皮细胞肿瘤坏死因子α的表达[J].实用医学杂志,2014,(20):3213-3215,3.基金项目
广西科学研究与技术开发计划项目（编号10124001A-32）；广西研究生教育创新计划 （）
