实用医学杂志Issue(20)：3219-3222,4.DOI:10.3969/j.issn.1006-5725.2014.20.006
miR-184在氧糖剥夺诱导SK-N-SH细胞缺血损伤中的作用及其对抗凋亡基因AKT2表达的调节
Role of miR-184 in oxygen-glucose-deprivation induced SK-N-SH cell ischemic injury and its regulation on AKT2 level
摘要
Abstract
Objective To explore the role of miR-184 in Oxygen-Glucose-Deprivation (OGD) induced SK-N-SH cell ischemic injury and its regulation on AKT2 level. Method We used a combination of oxygen and glucose deprivation to imitate ischemic conditions in vivo. MiR-184 mimic and inhibitor were transfected into SK-N-SH cell to alter miR-184 levels. The expression of miR-184 and AKT2 were determined by using Real-time PCR. The extent of SK-N-SH cell survival rate was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. Result Here, we observed that miR-184 was significantly inhibited in SK-N-SH cell after OGD (P<0.05). The changes of miR-184 level altered the expression of AKT2 mRNA. In addition, alteration of miR-184expressionsignificantly affected cell survival rate after OGD. Conclusion miR-184 plays an important role in ischemic injury through negatively regulating AKT2 level, which may provide a potential therapeutic target for ischemic stroke in miRNA levels.关键词
氧糖剥夺/miR-184/AKT2/缺血损伤Key words
Oxygen-glucose-deprivation/miR-184/AKT2/Ischemic injury引用本文复制引用
庞晓敏,向会尧,刘竞丽..miR-184在氧糖剥夺诱导SK-N-SH细胞缺血损伤中的作用及其对抗凋亡基因AKT2表达的调节[J].实用医学杂志,2014,(20):3219-3222,4.基金项目
国家自然科学基金 （）
