实用医学杂志Issue(23)：3735-3738,4.DOI:10.3969/j.issn.1006-5725.2014.23.010
P－糖蛋白在大鼠癫痫持续状态表达的动态变化及其意义
Significance and dynamic expression of P-glycoprotein in status epilepticus rats
摘要
Abstract
Objective To investigate the dynamic expression of the drug resistance protein P-glycoprotein (P-gp) within 72 hours in the pentylenetetrazol (PTZ)-induced status epilepticus (SE) model, and to identify the optimal detection time to inhibit P-gp. Methods mRNA and protein expressions of P-gp in rats hippocampal tissue were detected by using immunohistochemistry , RT-qPCR and Western blot at different time points after modeling (0, 3, 6, 12, 24, 48, 72 h). Results The mean density of P-gp protein in the hippocampus of status epilepticus model was 0.325 1 ± 0.008 2 at 24 h, and was 0.396 3 ± 0.016 8 at 48 h, which were consistently higher than those of the control group (P < 0.05, P < 0.01, respectively). Results of qRT-PCR showed that MDR1a expression was significantly upregulated at 24 h and at 48 h (P < 0.05, P < 0.01, respectively). Western blot assay revealed that P-gp protein was also significantly increased at 48 h after seizures (P < 0.05). Conclusions The upregulation of P-gp after SE peaked at 48 h, which maybe the optimal detection time to detect drug resistant after SE.关键词
癫痫持续状态/P-糖蛋白/戊四氮/耐药蛋白Key words
Status epilepticus/P-glycoprotein/Pentylenetetrazol/Drug Resistance protein引用本文复制引用
余涵,邓镇,罗淼珊,赵元淑,谢柳,陈丽,朱晓琴,胡景鑫,雷水生..P－糖蛋白在大鼠癫痫持续状态表达的动态变化及其意义[J].实用医学杂志,2014,(23):3735-3738,4.基金项目
广州市科技计划项目（编号2010YI-C891，11C32020697，2013J4100103）；广州市属高校科研计划项目 （）
