实用医学杂志Issue(5)：731-734,4.DOI:10.3969/j.issn.1006-5725.2015.05.012
广西百色地区结核分枝杆菌利福平和异烟肼耐药基因特征
The characteristics of the genes mutations in rifampin and isoniazid resistant Mycobacterium tuberculosis clinical isolates from Baise district, Guangxi autonomous region
摘要
Abstract
Objective To analyze the characteristics of the rpoB, KatG and inhA genes mutations in rifampin and isoniazid resistant Mycobacterium tuberculosis (MTB) clinical isolates in Baise district, Guangxi autonomous region. Methods 128 MTB clinical strains were collected and isolated for drug susceptibility testing, and drug resistant strain DNA was subtracted for rpoB, KatG and inhA genes mutation analysis. Results 75%(27/36)isolates carried mutations in the rpoB gene,and 59.3%(16/27)isolates carried mutations in 531 sites. 44.1%(15/34) isolates carried mutations in KatG or inhA, and 66.7%(10/15) isolates appeared in KatG 315 site, with two new mutations found in KatG 279 and 427 site. In these mutation isolates, 13.3%(2/15) mutations appeared in inhA 5, 6.7%(1/15) in inhA 16, and 20%(3/15) in both katG and inhA. Conclusions The mutation of rpoB, katG and inhA genes in TB is highly correlated with its resistance to rifampin and isoniazid in Baise district, Guangxi autonomous region. The study will provide a basis for further understanding the anti-bacterium mechanism and quick diagnostic methods for drug-resistant tuberculosis.关键词
结核/结核分枝杆菌/耐多药/异烟肼/利福平/rpoB/KatG/inhAKey words
Tuberculosis/Mycobacterium tuberculosis/Multidrug resistance/Isoniazid/Rifampin/rpoB/KatG/inhA引用本文复制引用
韦红玉,隆昕颖,凌俊,谢振锋,唐华英,黄小凤,韦连登,覃艳春,曾怡..广西百色地区结核分枝杆菌利福平和异烟肼耐药基因特征[J].实用医学杂志,2015,(5):731-734,4.基金项目
国家自然科学基金项目（编号81071345，81160535）；广西高等学校科研项目 （）
