实用医学杂志Issue(6)：999-1002,4.DOI:10.3969/j.issn.1006-5725.2015.06.044
广东EV71病毒流行病学特征和毒力分析
Epidemiological characteristics and virulence of EV71 in Guangdong province
摘要
Abstract
Objective To study the factors influencing epidemiological characteristics and virulence of Enterovirus 71 (EV71) in Guangdong province from 2008 to 2010. Methods RNA was extracted from collected samples or cultured virus , then reversing transcription into cDNA. We amplified full-length EV71-VP1 using poly-merase chain reaction technology , then conducted sequence alignment and established phylogenetic tree with MEGA software (version 5.0) to confirm the genotype of EV71. The association between severity of clinical symp-toms and sex, age, viral genotype and VP1 variation was also analyzed using Logistic regression. Results The genotype of the predominant epidemical strain was C4a in Guangdong from 2008 to 2010. However , this subtype had already differentiated into 4 subgroups (C4a1- C4a4). There was no correlation between clinical syndrome and sex or viral genotype; the severity of symptoms was negatively correlated with age: before 4 years old, varia-tion A289T can easily lead to severe cases, increasing the risk of infection (P<0.05, OR = 2.360, 95%CI:1.163~ 4.659). Conclusion The main epidemical EV71 strain is C4a1 in Guangdong province. The emerging differen-tiation and simultaneous prevalence should merit attention to strengthen relevant surveillance; and the protection of the susceptible population should be reinforced during EV71 prevalence.关键词
EV71病毒/毒力/流行病学特征Key words
Enterovirus 71/Virulence/Epidemiological Characteristics引用本文复制引用
吴娴波,钟艳云,曹宇娟,柯昌文,管大伟,张宝..广东EV71病毒流行病学特征和毒力分析[J].实用医学杂志,2015,(6):999-1002,4.基金项目
广东省科技计划项目（编号2012B031800132）；广东省医学科研基金 （）
