实用医学杂志Issue(7)：1088-1091,4.DOI:10.3969/j.issn.1006-5725.2015.07.015
APETx2对应激性胃黏膜损伤大鼠脊髓背根神经节酸敏感离子通道3表达的影响
Effect of APETx2 on the expression of ASIC3 in dorsal root ganglia in rat AGML model
摘要
Abstract
Objective To study the effect of APETx2 on the expression of ASIC3 APETx2 in a rat model of acute gastric mucosal lesion(AGML). Methods Twenty-four Wistar rats were randomly assigned to three groups in equal number : normal control group, water immersion restraint stress (WIRS) group, APETx2 treatment group. AGML was induced by WIRS for 6 hours, and APETx2 (25 μg/kg) was injected intraperitoneally before the onset of stress. Intragastric pH and gastric histopathological changes were measured and the expression of ASIC3 mRNA in DRG neurons projecting to rat stomach was examined by real-time PCR. Immunohistochemistry was performed to detect the localization of ASIC3. Results Compared with the normal control group, the WIRS group showed obvious gastric injury with lower values of intragastric pH and extensive expression of ASIC3 in the DRG neurons (P < 0.05). The treatment with APETx2 before the onset of WIRS significantly alleviated the gastric mucosal injury, decreased gastric acidity and reduced ASIC3 expression in DRG neurons (P < 0.05). Conclusions ASIC3 expression in DRG neurons projecting to rat stomach is strongly associated with gastric mucosal lesion and acidosis in the WIRS model. APETx2 can improve gastric acidosis and prevent the occurrence of these lesions.关键词
胃黏膜/APETx2/浸水束缚应激/大鼠/脊髓背根神经节/酸敏感离子通道3Key words
Gastric mucosa/APETx2/Water immersion restraint stress/Acid sensing ion channel 3/Dorsal root ganglia引用本文复制引用
徐少群,屠伟峰,郄文斌,温君琳,周红艳,蒋群,赵高峰..APETx2对应激性胃黏膜损伤大鼠脊髓背根神经节酸敏感离子通道3表达的影响[J].实用医学杂志,2015,(7):1088-1091,4.基金项目
国家自然科学基金项目（编号81272141）；广东省自然科学基金项目 （）
