实用医学杂志Issue(7)：1099-1101,3.DOI:10.3969/j.issn.1006-5725.2015.07.018
肾上腺切除幼鼠癫痫易感性及海马CRH mRNA的表达
Effect of bilateral adrenalectomy on the susceptibility to NMDA-induced seizure and hippocampal CRH mRNA in young rats
摘要
Abstract
Objective To demonstrate the effect of bilateral adrenalectomy (ADX) on the susceptibility to NMDA-induced seizure and hippocampal CRH mRNA in young rats. Methods 60 Wistar rats at P10 were divided into control group, ADX group and Sham-ADX group. In the next day after operation, 7 mg/kg NMDA was injected to induce seizure, and incubation period (in minutes) as well as degree was evaluated. In situ hybridization was used to detect hippocampal CRH mRNA expression. Results Latency was (43.65 ± 2.96) minutes in control group, (35.05 ± 2.35)minutes in ADX group and (42.60 ± 1.90)minutes in Sham-ADX group. Latency in the ADX rats increased significantly (F = 73.73, P < 0.05). The seizure scale was (4.40 ± 0.60) in control group, (5.56 ± 0.76) in ADX group and (4.55 ± 0.76) in Sham-ADX group. The severity of seizures in ADX group increased significantly compared with the control and Sham-ADX groups (F = 15.52, P <0.05). CRH mRNA expression was 20% in control group, 55% in ADX group and 15% in Sham-ADX group. CRH mRNA in the ADX group was significantly elevated (χ2 = 9.048, P < 0.05). Conclusion Adrenalectomy exacerbates NMDA-induced spasm seizures in young rats , which might be related to CRH mRNA expression in the hippocampus.关键词
痉挛,婴儿/肾上腺切除/肾上腺糖皮质激素释放激素/N-甲基-D-天冬氨酸Key words
Spasms, infantile/Adrenalectomy/Corticotrophin-releasing hormone/N-Methyl-D-aspartate引用本文复制引用
王雅洁,邹丽萍..肾上腺切除幼鼠癫痫易感性及海马CRH mRNA的表达[J].实用医学杂志,2015,(7):1099-1101,3.基金项目
国家重点基础研究发展计划支持（973）项目（编号2012cb517903）；国家自然科学基金项目（编号81471329）；北京市中医药科技项目 （）
