实用医学杂志Issue(11)：1838-1840,3.DOI:10.3969/j.issn.1006-5725.2015.11.037
蒲地蓝消炎口服液对呼吸道合胞病毒和腺病毒的体外抗病毒作用
Antiviral effects of pudilan xiaoyan oral liquid on respiratory syncytial virus and adenovirus in vitro
摘要
Abstract
Objective To study the antiviral effects of Pudilan xiaoyan oral liquid on Hep-2 cell models infected with respiratory syncytial virus (RSV), adenoviruses serotype 3 strains (ADV3) in vitro. Methods The cell cytotoxic and inhibition effect of Pudilan xiaoyan oral liquid on RSV or ADV3 were investigated by MTT assay and the inhibitory assay for cytopathic effect (CPE) in Hep-2 cell cultures to detect its antiviral effects. Results The median toxic concentration (TC50) of Pudilan xiaoyan oral liquid on Hep-2 cells was 776.97 mg/L. The median effective concentration (EC50) of inhibiting RSV and ADV3 were 28.08, 28.10 mg/L,whose therapeutic index (TI) were 27.67 and 27.65 respectively. The safety coefficient of Pudilan xiaoyan oral liquid is higher than that of control, ribavirin. Compared with the virus control group, Pudilan xiaoyan oral liquid can obviously produce actions of a dose-dependent relationship to inhibit CPE in Hep-2 cells infected with RSV or ADV3 virus. Conclusions Pudilan xiaoyan oral liquid significantly improves the protection against RSV and ADV3 virus infection in Hep-2 cells. And the inhibition of CPE induced by viruses infection increased with the elevation of higher drug concentration for its antiviral effect augmented in vitro.关键词
蒲地蓝消炎口服液/呼吸道合胞病毒/腺病毒3型/抗病毒作用Key words
Pudilan xiaoyan oral liquid/respiratory syncytial virus/adenoviruses serotype 3 strains/anti-viral effect引用本文复制引用
吴璇,于莉,胡涛,黄升海..蒲地蓝消炎口服液对呼吸道合胞病毒和腺病毒的体外抗病毒作用[J].实用医学杂志,2015,(11):1838-1840,3.基金项目
安徽省自然科学基金项目（编号1308085MH129）；安徽高校省级自然科学研究重点项目 （）
