实用医学杂志Issue(12)：1908-1911,4.DOI:10.3969/j.issn.1006-5725.2015.12.003
p53通过调控miR-148b抑制肺癌细胞PC-9的增殖
p53 inhibits the proliferation of lung cancer cell PC-9 by regulating miR-148b
摘要
Abstract
Objective To explore the function of p53 on regulating the expression of miR-148b in lung cancer cell line PC-9 and its corresponding molecular mechanism and the impact on cell proliferation. Methods Transient transfection of p53 eukaryotic expressing plasmids into lung cancer cell line PC-9 was performed to establish a cell model over-expressing p53. RT-PCR was used to explicit the impact of p53 on the expression of miR-148b. A reporter vector containing miR-148b promoter was used to investigate the function of p53 on regulating the transcription of miR-148b. Low-expressing miR-148b by transfecting its specific inhibitors , a CCK-8 assay was performed to explore the influence of miR-148b on the lung cancer cell proliferation inhibited by p53. Results Over-expression of p53 promoted miR-148b expression in lung cancer cell line PC-9. P53 could increase the luciferase activity driven by miR-148b promoters. Knockdown of miR-148b attenuated the impact of p53 on inhibiting the proliferation of PC-9 cells. Conclusion P53 inhibits the proliferation of lung cancer cell line PC-9 partially depending on miR-148b.关键词
肺肿瘤/p53/微小RNA-148b/细胞增殖Key words
Lung neoplasms/p53/miR-148b/Cell proliferation引用本文复制引用
傅寅佳,杨熹,来森艳,曹小年,王桂华,胡俊波,李襄..p53通过调控miR-148b抑制肺癌细胞PC-9的增殖[J].实用医学杂志,2015,(12):1908-1911,4.基金项目
国家973计划项目（编号2009CB521802）；国家自然科学基金资助项目（编号30973496）；国家青年科学基金项目 （）
