实用医学杂志Issue(13)：2115-2118,4.DOI:10.3969/j.issn.1006-5725.2015.13.015
自体脂肪间充质干细胞移植对糖尿病大鼠肾功能的改善作用
Autologous transplantation of adipose-derived mesenchymal stem cells improves nenal function in diabetic rats
摘要
Abstract
Objective To investigate the renoprotective effects of autologous transplantation of adipose-derived mesenchymal stem cells (ADMSCs) in diabetic rats. Methods Sprague-Dawley (SD) rats were injected intraperitoneally with 40 mg/kg streptozotocin (STZ) for 5 consecutive days to induce type 1 diabetes. Four weeks following STZ injection, eighteen SD rats were randomized into two groups: the diabetic group (n = 9) and the ADMSCs group (n = 9). Normal nondiaetic rats were set as the normal control (n = 9). Autologous ADMSCs were cultured and identified in vitro , which were intravenously injection to the ADMSCs group rats via the tail vein. At 8 weeks after transplantation, levels of blood glucose, insulin, serum urea nitrogen, serumcreatinine and urine protein were measured. Meanwhile the body weight and kidney weight were examined. Results Mesenchymal cell surface markers were expressed in the cultured ADMSCs. The ADMSCs could differentiate into the adipogenic and osteoblastic lineages. Both the diabetic group and the ADMSCs group rats had higher levels of blood glucose , urea nitrogen , serum creatinine , urine protein and higher ratio of the kidney weight/body weight than those in the normal control group (P < 0.05, respectively). Blood glucose, urea nitrogen and the ratio of kidney weight/body weight in the ADMSCs group rats were significantly decreased compared with the diabetic group (P < 0.05, respectively). The decreased insulin level was attenuated after transplantation of ADMSCs (P < 0.05). Besides, levels of serum creatinine and urine protein in the ADMSCs group were lower than those in the diabetic group with no significant difference. Conclusion Autologous transplantation of ADMSCs can improve metabolic disorder and relieves diabetic renal damage.关键词
糖尿病肾病/脂肪间充质干细胞/肾功能Key words
Diabetic nephropathy/Adipose-derived mesenchymal stem cells/Nenal function引用本文复制引用
闵小春,伍婷婷,杞少华,姚维琪,武栋成..自体脂肪间充质干细胞移植对糖尿病大鼠肾功能的改善作用[J].实用医学杂志,2015,(13):2115-2118,4.基金项目
武汉市卫计委科研基金资助项目 （）
