特产研究Issue(2)：5-8,4.
MSL抗菌肽对绿脓杆菌和金黄色葡萄球菌细胞膜的损伤作用研究
The Damage Effect of MSL Antimicrobial Peptide on the Membrane of the Pseudomonas Aeruginosa and Staphylococcus Aureus
摘要
Abstract
The aim of this study was to find the target of the MSL microbial peptide through The damage effect of MSL antimicrobial peptide on the membrane of the pseudomonas aeruginosa and staphylococcus aureus .MSL antimicrobial peptide was added into pseudomonas aeruginosa (1 × 108/mL) and staphylococcus aureus(1 × 108/mL) solution ,respectively .The final concentration of the solution was 1 × MIC .The solution was cultured at 37℃ .The supernatant was centrifuged and collected at 0min ,10min ,20min ,30min ,40min ,50min ,60min ,120min ,180min , 240min .The protein concentration was determined .MSL antimicrobial peptide was added into pseudomonas aeruginosa (1 × 108/mL ) and staphylococcus aureus(1 × 108/mL) solution ,respectively .The final concentration of the solution was 1 × MIC .The bacterial was cultured at 37℃ for 60min .The bacterial structure was observed by transmission electron microscopy .The result showed that cytosol released from the pseudomonas aeruginosa and staphylococcus aureus after the interaction of MSL antimicrobial peptide .The result of the transmission electron mi-croscopy showed that the MSL damaged the membrane of the pseudomonas aeruginosa and staphylococcus aureus ,electron density in the intracy-toplasm is decreased .Chromatin assemble .At the worst condition ,the structure of the membrane of the bacterial .It indicates that MSL damges the membrane of the G+ and G- bacterial and confirm that the membrane of the bacterial is the target of the MSL .关键词
抗菌肽/绿脓杆菌/金黄色葡萄球菌Key words
antimicriobial peptide/pseudomonas aeruginosa/staphylococcus aureus分类
生物科学引用本文复制引用
刘艳环,李海涛,朱言柱,张润祥,肖佳美,苗利光..MSL抗菌肽对绿脓杆菌和金黄色葡萄球菌细胞膜的损伤作用研究[J].特产研究,2015,(2):5-8,4.基金项目
吉林省科技厅发展计划 （）
