特产研究Issue(2)：23-29,7.
人参、西洋参不同部位中齐墩果酸型皂苷含量的对比分析
Profiling and Identification of Oleanolic Acid Type Saponins in Different Parts of Panax ginseng C.A.Mey .and Panax quinquefolium L
摘要
Abstract
To observe the distribution of Oleanolic Acid Type Saponins in ginseng and American ginseng ,we analyzed the species of oleanolic acid type saponins and the amount of ginsenoside Ro in roots ,stems and leaves of both of them with high performance liquid chromatography tandem mass spectrometry .Column:Agilent TC-C18 (4.6mm × 250mm ,5μm);The mobile phase consisted of two eluents:Acetonitrile and wa-ter with 2.5mmol/L Ammonium acetate(w/v) and 0.05‰ Ammonia(v/v);Gradient elution;The flow rate was 1mL/min;203nm as detection wavelength .Six compounds of oleanolic acid type saponins were identified .They were ginsenoside Ro(1) ,chikusetsusaponin Iva(2) ,zingibro-side R1 (3) ,Stipulenaoside R2 (4) ,Arrnatoslde(5) and Elatoside K(6) .The compound 1 ,2 and 3 were all found in root ,stem and leaf of gin-seng .About american ginseng ,compound 1 ,3 and 4 were in stem ,compound 1 and 4 were in leaf .And above all six compounds were in root . Root is a main storage part of ginsenoside Ro .The contents of ginsenoside Ro in American ginseng root(0.576% ) were more than in ginseng root(0.214% ) .The amounts of ginsenosides Ro in different parts from both ginsengs were root> stem> leaf .(conclusion )The Species of oleanolic acid type saponins are different in various parts of Panax ginseng and American ginseng .But the distribution of ginsenoside Ro are similar in both ginsengs .The roots are the main part for accumulation of oleanolic acid type saponins .关键词
人参/西洋参/人参皂苷Ro/齐墩果酸型皂苷/高效液相色谱质谱联用Key words
Panax ginseng C.A.Mey ./Panax quinquefolium L ./gingsenoside Ro/oleanolic acid type saponins/HPLC-MS/MS分类
医药卫生引用本文复制引用
王佳,郑培和,许世泉,刘敏,王英平..人参、西洋参不同部位中齐墩果酸型皂苷含量的对比分析[J].特产研究,2015,(2):23-29,7.基金项目
国家科技支撑计划项目（2011BAI03B0106）；吉林省医药产业发展专项资金项目 （）
