天津科技大学学报Issue(4)：1-5,5.DOI:10.13364/j.issn.1672-6510.2014.04.001
大鼠胚胎神经干细胞体外培养及诱导分化
Culture and Differentiation of Rat Embryonic Neural Stem Cells in Vitro
摘要
Abstract
Neural stem cells(NSCs)have great potential in basic research and clinical application. In this study,NSCs were isolated from the forebrain of SD rat embryos,cultured in vitro,and then induced to differentiate by removing the growing factors or adding all-trans retinoic acid(ATRA). Immunofluorescence technology was used to test the expression of Nestin and differentiation markerβ-Ⅲtubulin and GFAP,and then the percentage of differentiated cells were calculated. The results show that the isolated cells can be amplified in vitro and exhibit Nestin positive expression. The differentiated cells expressedβ-Ⅲtubulin and GFAP. Compared with growing factor withdrawal,addition of ATRA resulted in moreβ-Ⅲtubulin positive cells.关键词
神经干细胞/SD大鼠胚胎/扩增/分化Key words
neural stem cells/SD rat embryo/proliferation/differentiation分类
生物科学引用本文复制引用
王楠,李晓凡,庄春雨,徐尧,菅若蕾,张同存..大鼠胚胎神经干细胞体外培养及诱导分化[J].天津科技大学学报,2014,(4):1-5,5.基金项目
国家自然科学基金资助项目(31171303)；教育部科技研究重点项目(212010) （31171303）
