天津科技大学学报Issue(4)：6-10,5.DOI:10.13364/j.issn.1672-6510.2014.04.002
不同亚型人SMYD3启动子转录活性比较
Comparison of Transcriptional Activities of Different Human SMYD3 Isoforms
摘要
Abstract
In this study,promoters of two isoforms were cloned with PCR method and then their luciferase reporter plas-mids were constructed. Using the method of Luciferase Assay,the transcriptional activities of these two promotors of SMYD3 were detected in COS-7 cells. Results showed that the transcriptional activity of the promoter of isoform 1 is higher that that of the promoter of isoform 2. Bioinformatics analyses indicated that the significant difference in the transcriptional activity between these two promoters might be due to the tandem repeats of E2F-1 binding sites located in the promoter of isoform 1.关键词
SMYD3/启动子/转录活性Key words
SMYD3/promotor/transcriptional activity分类
生物科学引用本文复制引用
刘磊,罗学刚,赵文文,穆爱,辛中帅,张同存..不同亚型人SMYD3启动子转录活性比较[J].天津科技大学学报,2014,(4):6-10,5.基金项目
国家自然科学基金资助项目(31000343，31300642，31071126) （31000343，31300642，31071126）
