天津科技大学学报Issue(5)：1-5,5.DOI:10.13364/j.issn.1672-6510.2014.05.001
运用绿色荧光蛋白探讨肉葡萄球菌双精氨酸分泌途径
GFP Translocation of Twin-arginine Secretion Pathway in Staphylococcus carnosus
摘要
Abstract
According to the genome sequence of Staphylococcus carnosus TM300 genome,a pair of primers was designed for PCR amplification of promoter Peftu of tufA gene. The PCR-amplified promoter Peftu fragment was cloned into plasmid pBT2-Tat-GFP and chemically transformed into E. coli host. A shuttle vector pBT2-ETG was thereby constructed and succ-essfully transformed into E. coli and S. carnosus TM300 hosts,respectively. The experimental results reveal that GFP was expressed by the E. coli-Staphylococcus shuttle vector pBT2-ETG in both E. coli and S. carnosus hosts. The expressed GFP was translocated to the cell walls of S. carnosus host in fluorescent active form. Thereby,a preliminary experimental basis was laid for the further study of other exogenous protein secretion via twin-arginine translocation pathway in S. carnosus.关键词
肉葡萄球菌/绿色荧光蛋白(GFP)/双精氨酸转运途径/启动子Peftu/Tat信号肽Key words
Staphylococcus carnosus/GFP/twin-arginine translocation pathway/promoter Peftu/Tat signal peptide分类
生物科学引用本文复制引用
高强,许保银,程逸冰,王林,张朝正,田萍萍..运用绿色荧光蛋白探讨肉葡萄球菌双精氨酸分泌途径[J].天津科技大学学报,2014,(5):1-5,5.基金项目
国家自然科学基金资助项目(31370075，31101275)；国家高技术研究发展计划“863计划”资助项目(2012AA021302) （31370075，31101275）
