天津科技大学学报Issue(6)：32-35,40,5.DOI:10.13364/j.issn.1672-6510.2014.06.007
利用木糖母液生产碱性蛋白酶发酵工艺的初步研究
Alkaline Protease Fermentation Process Using Xylose Mother Liquor
摘要
Abstract
In this study,engineered Bacillus licheniformis TCCC11843,was used for the fermentation production of alkaline protease from a carbon source:xylose mother liquor,a by-product of xylose production. The optimized fermentation pa-rameters of alkaline protease production were:inoculum culture age was 12,h,initial pH 6.5,inoculum volume 8%, fermentation temperature 34,℃,and feeding 4.0,mL 50%xylose mother liquor at the 24th,h. Under such optimal fermenta-tion conditions,the maximum enzyme activity can reach 7,838,U/mL. Moreover,feedback feeding experiments using the 7,L fermenter resulted in the maximum enzyme activity of 14,912,U/mL at 80,h. These results are valuable for the optimal com-mercial production of alkaline protease.关键词
木糖母液/地衣芽孢杆菌/碱性蛋白酶/补料发酵Key words
xylose mother liquor/Bacillus licheniformis/alkaline protease/fed-batch fermentation分类
生物科学引用本文复制引用
卜令军,李玉,路福平,王正祥..利用木糖母液生产碱性蛋白酶发酵工艺的初步研究[J].天津科技大学学报,2014,(6):32-35,40,5.基金项目
国家高技术研究发展计划“863计划”资助项目(2011AA100905-4)；教育部长江学者和创新团队发展计划资助项目(IRT1166) （2011AA100905-4）
