天津科技大学学报Issue(3)：9-13,5.DOI:10.13364/j.issn.1672-6510.20140097
玉米芯诱导里氏木霉Rut C30产纤维素酶的分析
Analysis of Cellulase Production withTrichoderma reeseiRut C30 Induced by Corn Cob
摘要
Abstract
Trichoderma reesei Rut C30 was induced by different lignocellulosic inducers with the same concentration of 10,g/L to produce enzymes,and the enzyme-producing curves were determined. Results indicated that the production of cel-lulase,β-glucosidase and xylanase were the highest when corn cob was used as the inducing carbon source,and the produc-tion was up to 1.53,FPU/mL,0.61 IU/mL and 72.86 IU/mL respectively after inducing 120 hours. The sugar composition and variation during the enzymatic hydrolysis of corn cob were analyzed with high performance liquid chromatography(HPLC). Monosaccharide detected included glucose,xylose,arabinose,mannose and galactose. The results of induction experiments indicated that only galactose among monosaccharides could induce cellulase.关键词
玉米芯/里氏木霉/纤维素酶/诱导Key words
corn cob/Trichoderma reesei/cellulase/induction分类
生物科学引用本文复制引用
马立娟,蔡瑞,崔有志,赫荣琳,陈树林,肖冬光..玉米芯诱导里氏木霉Rut C30产纤维素酶的分析[J].天津科技大学学报,2015,(3):9-13,5.基金项目
国家自然科学基金资助项目(21106102) （21106102）
